Interaction between Human Respiratory Syncytial Virus (RSV) M2-1 and P Proteins Is Required for Reconstitution of M2-1-Dependent RSV Minigenome Activity

Interaction between human respiratory syncytial virus (RSV) M2-1 and P proteins is required for reconstitution of M2-1-dependent RSV minigenome activity.

We have investigated protein-protein interactions among the respiratory syncytial virus (RSV) RNA polymerase subunits using affinity chromatography. Here we demonstrate a novel interaction of P and M2-1 proteins. Phosphorylation of either M2-1 or P appears to be dispensable for this interaction. Internal deletions within P mapped the M2-1-binding domain to a region between residues 100 and 120....

What is respiratory syncytial virus (RSV)?

one of the most common causes of bronchiolitis and pneumonia in children under 1 year of age in the United States. It is a common reason for young infants to be hospitalized during the RSV season. RSV can also cause serious respiratory illness in older adults. Most healthy people recover from RSV infection in 1 to 2 weeks. However, infection can be severe in a few, usually among those who are a...

Respiratory Syncytial Virus (RSV) Infection

Identification of amino acids that are critical to the processivity function of respiratory syncytial virus M2-1 protein.

The M2-1 protein of respiratory syncytial virus (RSV) is a transcription processivity factor that is essential for virus replication. The function of RSV M2-1 protein can be examined by using an RSVlacZ minigenome assay in vitro since the expression of the lacZ gene is dependent on M2-1. The M2-1 protein of pneumonia virus of mice (PVM), also a member of the Pneumovirus genus, functions poorly ...

Structural phosphoprotein M2-1 of the human respiratory syncytial virus is an RNA binding protein.

The structural phosphoprotein M2-1 of human respiratory syncytial virus (HRSV) Long strain shows RNA binding capacity in three different assays that detect RNA-protein complexes: cross-linking, gel retardation, and Northern-Western assays. It is able to bind HRSV leader RNA specifically with cooperative kinetics, with an apparent K(d) of at least 90 nM. It also binds to long RNAs with no sequen...